Human Macrophage Derived Chemokine (MDC) ELISA Kit

Cat.#:

JL19541

Regulatory Status:

RUO

Alternative:

CCL22; ABCD1; DC/B-CK; SCYA22; STCP1; Chemokine C-C-Motif Ligand 22; Small Inducible Cytokine Subfamily A(Cys-Cys)Member 22; Stimulated T-Cell Chemotactic Protein 1

Detection Method:

ELISA

Assay Type:

Sandwich

Detection Range:

78.12-5000pg/mL

Sensitivity:

31.56pg/mL

Specificity:

This kit recognizes Human MDC in samples. No significant cross-reactivity or interference between Human MDC and analogues was observed.

Sample Type:

serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Assay Procedure:

Research Area:

Immunology

Size & Price:

Human A1BG Polyclonal Antibody

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Product Datasheets

Instruction:

Data
Citations
Sequence / 3D

| MDC

C-C motif chemokine 22 is a protein that is encoded by the CCL22 gene.The protein encoded by this gene is secreted by dendritic cells and macrophages, and elicits its effects on its target cells by interacting with cell surface chemokine receptors such as CCR4.The gene for CCL22 is located in human chromosome 16 in a cluster with other chemokines called CX3CL1 and CCL17.

| Typical data

As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, wash technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data are provided below for reference only.

Concentration (pg/mL)	5000	2500	1250	625	312.5	156.25	78.12	0
OD	1.98	1.1	0.71	0.49	0.31	0.21	0.17	0.07
Corrected OD	1.91	1.03	0.64	0.42	0.24	0.14	0.1	-

| Repeatability

Intra-assay Precision (Precision within an assay)：CV% < 10%; Inter-assay Precision (Precision between assays)：CV% < 10%

| Recovery

The recovery of Human MDC spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type	Range(%)	Average Recovery(%)
Serum(n=8)	84-101	96
Plasma(n=8)	92-107	104
Cell Culture Supernates(n=8)	96-108	105

| Linearity

Samples were spiked with high concentrations of Human MDC and diluted with Universal Diluent to produce samples with values within the range of the assay.

		Serum(n=4)	Plasma(n=4)	Cell Culture Supernates(n=4)
1 : 2	Range (%)	84-95	86-96	90-110
1 : 2	Average (%)	91	91	96
1 : 4	Range (%)	89-103	87-108	105-115
1 : 4	Average (%)	94	98	108